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Canadian Mouse Mutant Repository
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Protocols  
 

Germ Cell Tissue Archiving

Embryos, sperm, and ovaries are collected and frozen in liquid nitrogen from ENU and Non-ENU mice. The various protocols are constantly updated according to the most effective method so that optimal results are obtained.

Embryo Cryopreservation & Thaw
Ovary Cryopreservation (PDF)
Sperm Cryopreservation (PDF)
Post-Thaw Sperm Evaluation (PDF)
In Vitro Fertilization (Unassisted IVF) (PDF)
Intracytoplasmic Sperm Injection (ICSI - Assisted IVF) (PDF)
Media Preparation

The Hospital for Sick Children
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