Glossary

Many phrases and terms are used in the policies and procedures. Please refer to the following list for clarification.

CMHD	Centre for Modeling Human Disease
CMMR	Canadian Mouse Mutant Repository
DI	Depositing Investigator
	The researcher or institution submitting mutants or samples to the CMMR.
ENU	N-ethyl-N-nitrosourea
ES Cell	Embryonic Stem Cell
	Alkylating mutagen that induces random point mutations.
FIMRe	Federation of International Mouse Repositories
GT	Gene Trap
ICSI	Intracytoplasmatic Sperm Injection
IGTC	International Gene Trap Consortium
LIMS	Laboratory Information Management System
IMSR	International Mouse Strain Resource
	The TCP web application used by investigators to request services available at http://lims.phenogenomics.ca
IS	Inbred Strain (congenic)
	A group of organisms so highly inbred as to be genetically identical, except for sexual differences. Brother sister matings for 20 or more generations are required before a strain can be defined as ‘inbred’.
IVF	In Vitro Fertilization
	The process by which mature oocytes from superovulated female mice are fertilized in a Petri dish with epididymal sperm. Rescue effort for ill, aged unproductive males.
KO	Knock Out
	A mutant mouse containing a null allele for a gene under study, generated by homologous recombination in embryonic stem cells.
MFGC	Mammalian Functional Genomics Centre
MGI	Mouse Genome Informatics
MMRRC	Mutant Mouse Regional Resource Center
MTA	Material Transfer Agreement
	Agreement authorizes transfers of mice or cryopreserved samples between the donating investigator and the CMMR (Donor MTA) and the CMMR and requesting investigator (Requesting MTA).
NorCOMM	North American Conditional Mouse Mutagenesis project
	Ovarian Transplantation
	Generation of offspring using cryopreserved ovaries. Rescue effort perfomed for aged, ill or unproductive females.
PCR	Polymerase Chain Reaction
PI	Principal Investigator
	The senior researcher of an institution responsible for the project.
	Recovery
	The freezing of sperm and/or ovaries as reagents of a mutant strain.
	Rederivation
	The transfer of frozen or fresh embryos for transplantation in recipient females in order to generate a specific strain.
RACE	reverse transcription and amplification of cDNA ends
	Sperm Assessment
	Comprehensive sperm analysis that includes sperm concentration, sperm motility and sperm viability.
SOP	Standard Operating Protocol
TCP	Toronto Centre for Phenogenomics
TG	Transgenics
	Animals into which cloned genetic material has been experimentally transferred.
WI	Withdrawing Investigator / Institution
	The researcher or institution requesting a sample from the CMMR.